2D electrophoretic profile of Fasciola hepatica intestine proteins

Marco Cabrera-González; Diana Marlo-Javier; Carlos Quilcate-Pairazamán; Héctor V. Vásquez; Medali Cueva-Rodríguez

doi:10.52973/rcfcv-e32103

2D electrophoretic profile of Fasciola hepatica intestine proteins

Marco Cabrera-González
, Diana Marlo-Javier
, Carlos Quilcate-Pairazamán
, Héctor V. Vásquez
, Medali Cueva-Rodríguez

Research output: Contribution to journal › Article › peer-review

Abstract

Being fasciolosis an important parasitic infection in ruminants in many Countries and given the high prevalence in humans and animals in Cajamarca, Peru. It was proposed to carry out the study on the profile of the intestinal proteins of Fasciola hepatica with the objective of knowing the number of proteins and the pH range of secretion/ excretion of the intestine of the parasite that it expresses by means of the 2D-bidimensional electrophoresis method. Adult samples of F. hepatica were collected from bovine livers in the Cajamarca Municipal Slaughterhouse. They were transferred to the Animal Health Biotechnology Laboratory of the Baños del Inca Agrarian Experimental Station, INIA – Cajamarca, for processing. The electrophoretic run allowed to separate 82 proteins with different molecular weights, focused at different isoelectric points in a pH range of 6.0 – 9.4. It is concluded that by analyzing the 2D gel of F. hepatica gut proteins, 84 protein spots with different molecular weights were identified, focused on different isoelectric points in a range of 6.0 – 9.4.

Translated title of the contribution	Perfil electroforético 2D de las proteínas del intestino de Fasciola hepatica
Original language	English
Article number	e32103
Journal	Revista Cientifica de la Facultad de Veterinaria
Volume	32
DOIs	https://doi.org/10.52973/rcfcv-e32103
State	Published - 1 Jan 2022

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title = "2D electrophoretic profile of Fasciola hepatica intestine proteins",

abstract = "Being fasciolosis an important parasitic infection in ruminants in many Countries and given the high prevalence in humans and animals in Cajamarca, Peru. It was proposed to carry out the study on the profile of the intestinal proteins of Fasciola hepatica with the objective of knowing the number of proteins and the pH range of secretion/ excretion of the intestine of the parasite that it expresses by means of the 2D-bidimensional electrophoresis method. Adult samples of F. hepatica were collected from bovine livers in the Cajamarca Municipal Slaughterhouse. They were transferred to the Animal Health Biotechnology Laboratory of the Ba{\~n}os del Inca Agrarian Experimental Station, INIA – Cajamarca, for processing. The electrophoretic run allowed to separate 82 proteins with different molecular weights, focused at different isoelectric points in a pH range of 6.0 – 9.4. It is concluded that by analyzing the 2D gel of F. hepatica gut proteins, 84 protein spots with different molecular weights were identified, focused on different isoelectric points in a range of 6.0 – 9.4.",

keywords = "2D electrophoresis, Bovine, fasciolosis, parasites",

author = "Marco Cabrera-Gonz{\'a}lez and Diana Marlo-Javier and Carlos Quilcate-Pairazam{\'a}n and V{\'a}squez, \{H{\'e}ctor V.\} and Medali Cueva-Rodr{\'i}guez",

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doi = "10.52973/rcfcv-e32103",

language = "English",

volume = "32",

journal = "Revista Cientifica de la Facultad de Veterinaria",

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T1 - 2D electrophoretic profile of Fasciola hepatica intestine proteins

AU - Cabrera-González, Marco

AU - Marlo-Javier, Diana

AU - Quilcate-Pairazamán, Carlos

AU - Vásquez, Héctor V.

AU - Cueva-Rodríguez, Medali

PY - 2022/1/1

Y1 - 2022/1/1

N2 - Being fasciolosis an important parasitic infection in ruminants in many Countries and given the high prevalence in humans and animals in Cajamarca, Peru. It was proposed to carry out the study on the profile of the intestinal proteins of Fasciola hepatica with the objective of knowing the number of proteins and the pH range of secretion/ excretion of the intestine of the parasite that it expresses by means of the 2D-bidimensional electrophoresis method. Adult samples of F. hepatica were collected from bovine livers in the Cajamarca Municipal Slaughterhouse. They were transferred to the Animal Health Biotechnology Laboratory of the Baños del Inca Agrarian Experimental Station, INIA – Cajamarca, for processing. The electrophoretic run allowed to separate 82 proteins with different molecular weights, focused at different isoelectric points in a pH range of 6.0 – 9.4. It is concluded that by analyzing the 2D gel of F. hepatica gut proteins, 84 protein spots with different molecular weights were identified, focused on different isoelectric points in a range of 6.0 – 9.4.

AB - Being fasciolosis an important parasitic infection in ruminants in many Countries and given the high prevalence in humans and animals in Cajamarca, Peru. It was proposed to carry out the study on the profile of the intestinal proteins of Fasciola hepatica with the objective of knowing the number of proteins and the pH range of secretion/ excretion of the intestine of the parasite that it expresses by means of the 2D-bidimensional electrophoresis method. Adult samples of F. hepatica were collected from bovine livers in the Cajamarca Municipal Slaughterhouse. They were transferred to the Animal Health Biotechnology Laboratory of the Baños del Inca Agrarian Experimental Station, INIA – Cajamarca, for processing. The electrophoretic run allowed to separate 82 proteins with different molecular weights, focused at different isoelectric points in a pH range of 6.0 – 9.4. It is concluded that by analyzing the 2D gel of F. hepatica gut proteins, 84 protein spots with different molecular weights were identified, focused on different isoelectric points in a range of 6.0 – 9.4.

KW - 2D electrophoresis

KW - Bovine

KW - fasciolosis

KW - parasites

UR - https://www.scopus.com/pages/publications/85172916426

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DO - 10.52973/rcfcv-e32103

M3 - Article

AN - SCOPUS:85172916426

SN - 0798-2259

VL - 32

JO - Revista Cientifica de la Facultad de Veterinaria

JF - Revista Cientifica de la Facultad de Veterinaria

M1 - e32103

ER -

2D electrophoretic profile of Fasciola hepatica intestine proteins

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