TY - JOUR
T1 - Effect of Different Extraction Solvents on the Total Phenolic Content and Antioxidant Activity of Brassica oleracea var. italica
AU - Florian, Vivian Nathaly Echegaray
AU - Sisniegas, Claudia Giuliana Montoya
AU - Valdiviezo-Campos, Juan Ernesto
N1 - Publisher Copyright:
© 2025 Phcogj.Com.
PY - 2025/1/1
Y1 - 2025/1/1
N2 - This study offers an alternative solution for the prevention and/or treatment of diseases caused by free radicals. The objective was to evaluate the effect of different solvents on the total phenolic content and antioxidant activity of Brassica oleracea var. italica (broccoli). Methods: The sample, consisting of stems and florets of Brassica oleracea var. italica (broccoli), were collected from the Chocas community in Carabayllo, Lima, Peru. Three extracts were prepared using different solvents, including a mixture of ethanol and water. The total phenolic content was determined using the Folin-Ciocalteu method, and antioxidant capacity was evaluated using two specific assays (DPPH and ABTS). Additionally, the correlation between total phenolic content and antioxidant activity was analyzed. Results: The hydroethanolic extract demonstrated the highest phenolic content, with 686.02 mg GAE/100 g dry matter). It also exhibited strong antioxidant activity, measuring 1035.81 mg TE/100 g DM in the DPPH assay and 6506.94 mg TE/100 g DM in the ABTS assay. Conclusion: The highest total phenolic content and antioxidant activity of Brassica oleracea var. italica were found in the hydroethanolic extract, which showed a significant high correlation. Frequent consumption of broccoli in the diet is recommended due to its high values.
AB - This study offers an alternative solution for the prevention and/or treatment of diseases caused by free radicals. The objective was to evaluate the effect of different solvents on the total phenolic content and antioxidant activity of Brassica oleracea var. italica (broccoli). Methods: The sample, consisting of stems and florets of Brassica oleracea var. italica (broccoli), were collected from the Chocas community in Carabayllo, Lima, Peru. Three extracts were prepared using different solvents, including a mixture of ethanol and water. The total phenolic content was determined using the Folin-Ciocalteu method, and antioxidant capacity was evaluated using two specific assays (DPPH and ABTS). Additionally, the correlation between total phenolic content and antioxidant activity was analyzed. Results: The hydroethanolic extract demonstrated the highest phenolic content, with 686.02 mg GAE/100 g dry matter). It also exhibited strong antioxidant activity, measuring 1035.81 mg TE/100 g DM in the DPPH assay and 6506.94 mg TE/100 g DM in the ABTS assay. Conclusion: The highest total phenolic content and antioxidant activity of Brassica oleracea var. italica were found in the hydroethanolic extract, which showed a significant high correlation. Frequent consumption of broccoli in the diet is recommended due to its high values.
KW - Antioxidant
KW - Broccoli
KW - Extract
KW - Polyphenol
KW - Vegetable
UR - http://www.scopus.com/inward/record.url?scp=86000336522&partnerID=8YFLogxK
U2 - 10.5530/pj.2025.17.7
DO - 10.5530/pj.2025.17.7
M3 - Article
AN - SCOPUS:86000336522
SN - 0975-3575
VL - 17
SP - 58
EP - 62
JO - Pharmacognosy Journal
JF - Pharmacognosy Journal
IS - 1
ER -