TY - JOUR
T1 - 2D electrophoretic profile of Fasciola hepatica intestine proteins
AU - Cabrera-González, Marco
AU - Marlo-Javier, Diana
AU - Quilcate-Pairazamán, Carlos
AU - Vásquez, Héctor V.
AU - Cueva-Rodríguez, Medali
N1 - Publisher Copyright:
© (2022). All Rights Reserved.
PY - 2022/1/1
Y1 - 2022/1/1
N2 - Being fasciolosis an important parasitic infection in ruminants in many Countries and given the high prevalence in humans and animals in Cajamarca, Peru. It was proposed to carry out the study on the profile of the intestinal proteins of Fasciola hepatica with the objective of knowing the number of proteins and the pH range of secretion/ excretion of the intestine of the parasite that it expresses by means of the 2D-bidimensional electrophoresis method. Adult samples of F. hepatica were collected from bovine livers in the Cajamarca Municipal Slaughterhouse. They were transferred to the Animal Health Biotechnology Laboratory of the Baños del Inca Agrarian Experimental Station, INIA – Cajamarca, for processing. The electrophoretic run allowed to separate 82 proteins with different molecular weights, focused at different isoelectric points in a pH range of 6.0 – 9.4. It is concluded that by analyzing the 2D gel of F. hepatica gut proteins, 84 protein spots with different molecular weights were identified, focused on different isoelectric points in a range of 6.0 – 9.4.
AB - Being fasciolosis an important parasitic infection in ruminants in many Countries and given the high prevalence in humans and animals in Cajamarca, Peru. It was proposed to carry out the study on the profile of the intestinal proteins of Fasciola hepatica with the objective of knowing the number of proteins and the pH range of secretion/ excretion of the intestine of the parasite that it expresses by means of the 2D-bidimensional electrophoresis method. Adult samples of F. hepatica were collected from bovine livers in the Cajamarca Municipal Slaughterhouse. They were transferred to the Animal Health Biotechnology Laboratory of the Baños del Inca Agrarian Experimental Station, INIA – Cajamarca, for processing. The electrophoretic run allowed to separate 82 proteins with different molecular weights, focused at different isoelectric points in a pH range of 6.0 – 9.4. It is concluded that by analyzing the 2D gel of F. hepatica gut proteins, 84 protein spots with different molecular weights were identified, focused on different isoelectric points in a range of 6.0 – 9.4.
KW - 2D electrophoresis
KW - Bovine
KW - fasciolosis
KW - parasites
UR - https://www.scopus.com/pages/publications/85172916426
U2 - 10.52973/rcfcv-e32103
DO - 10.52973/rcfcv-e32103
M3 - Article
AN - SCOPUS:85172916426
SN - 0798-2259
VL - 32
JO - Revista Cientifica de la Facultad de Veterinaria
JF - Revista Cientifica de la Facultad de Veterinaria
M1 - e32103
ER -