TY - GEN
T1 - Biodegradation of n-alkanes by Pseudomonas aeruginosa and Actinobacterial (Indigenous Bacteria) in Bilge Water
AU - Cabello-Torres, Rita
AU - Yaya-Chileno, Leslie
AU - Alegría-Amedo, María C.
AU - Romero-Cabello, Edison A.
AU - Castańeda-Olivera, Carlos
AU - Valdiviezo-Gonzales, Lorgio
N1 - Publisher Copyright:
© 2023 IEEE.
PY - 2023/1/1
Y1 - 2023/1/1
N2 - The objective of this study was to evaluate the degradation efficiency of n-alkanes by a mixture of two Gram-positive strains and one Gram-negative strain. The methodology consisted in adapting an inoculum of bilge water, in an aqueous solution enriched with petroleum diesel, nutrients, and air for 15 days at 22°C. Then, 03 indigenous strains were isolated and identified, two Gram-positive bacteria were mixed and the Gram-negative strain was separated. Each group was inoculated separately into aerated reactors containing bilge water for 7 days. Finally, the C5-C10, C10-C28, and C25-C40 fractions were quantified by gas chromatography-FID to calculate the removal achieved. The results showed the presence of Rhodococcus spp., Micrococcus spp., and Pseudomona aeruginosa. These indigenous bacteria produced emulsions with each treatment. Pseudomona aeruginosa removed 91.9% of the short chains (C5-C10) and 86% of the long chains (C10-C40). While Actinobacteria increased the removal from 55.3% (C5-C10) to 65% (C10-C40). This study shows the potential of these indigenous bacteria to remove n-alkanes from bilge water.
AB - The objective of this study was to evaluate the degradation efficiency of n-alkanes by a mixture of two Gram-positive strains and one Gram-negative strain. The methodology consisted in adapting an inoculum of bilge water, in an aqueous solution enriched with petroleum diesel, nutrients, and air for 15 days at 22°C. Then, 03 indigenous strains were isolated and identified, two Gram-positive bacteria were mixed and the Gram-negative strain was separated. Each group was inoculated separately into aerated reactors containing bilge water for 7 days. Finally, the C5-C10, C10-C28, and C25-C40 fractions were quantified by gas chromatography-FID to calculate the removal achieved. The results showed the presence of Rhodococcus spp., Micrococcus spp., and Pseudomona aeruginosa. These indigenous bacteria produced emulsions with each treatment. Pseudomona aeruginosa removed 91.9% of the short chains (C5-C10) and 86% of the long chains (C10-C40). While Actinobacteria increased the removal from 55.3% (C5-C10) to 65% (C10-C40). This study shows the potential of these indigenous bacteria to remove n-alkanes from bilge water.
KW - Actinobacteria
KW - Pseudomona aeruginosa
KW - biodegradation
KW - biosurfactants
KW - indigenous bacteria
KW - n-alkanes
UR - https://www.scopus.com/pages/publications/85179520756
U2 - 10.1109/ETCM58927.2023.10308806
DO - 10.1109/ETCM58927.2023.10308806
M3 - Conference contribution
AN - SCOPUS:85179520756
T3 - ECTM 2023 - 2023 IEEE 7th Ecuador Technical Chapters Meeting
BT - ECTM 2023 - 2023 IEEE 7th Ecuador Technical Chapters Meeting
A2 - Lalaleo, David Rivas
A2 - Chauvin, Manuel Ignacio Ayala
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 7th IEEE Ecuador Technical Chapters Meeting, ECTM 2023
Y2 - 10 October 2023 through 13 October 2023
ER -